Transcription Protease Synthase Discovered by Strain Solibacillus Sylvestris

Abstract

The goal of this work is to maximise the synthesis of bacterial alkaline protease from a mangrove isolate, Solibacillus silvestris, which has the capacity to create valuable primary and secondary metabolites of biotechnological value. Methodology: Using MALDI-TOF (mass spectrometry), the bacterium (known as Madhwad 103 Summer Zobell) used in the current study was isolated from soil that was obtained from Madhwad, Gir, India, during the summer. It was subsequently examined, utilising various physical and chemical variables, since it exhibited the greatest alkaline protease enzyme activity of the isolates. Selected parameters, i.e., Response Surface Methodology (RSM), were also applied to the optimisation of sucrose, peptone, and salinity. Results: Solibacillus silvestris was identified as the bacterium, which was given the name Madhwad 10-3 Summer Zobell. The results showed that the highest enzyme production can be achieved at pH 8.5, 40 °C of temperature, 48 hours of incubation, and an agitation speed of 120 rpm, while the highest enzyme activity was found to be given by sucrose and peptone with 0% salinity, with values of 221.7 U/ml and 246.7 U/ml, respectively. All the factors were significant in the statistical analysis carried out using RSM, which produced the maximum result of 258.750 U/ml at 2.5% sucrose, 10% peptone, and 0% salinity. Applications and improvements: The enzyme created as a result of its effectiveness at operating at an alkaline pH can be used in bioremediation as well as the detergent and textile industries.