A Setting Technique for Comparative Protein Modelling for Web based SMART Tool
DOI:
https://doi.org/10.5281/zenodo.10691316Keywords:
smart tool, protein, pssm, aluncAbstract
When the "hairless protein" linked to the hairless gene, which is necessary for hair growth, stops working, the result will be total hairlessness. This gene is located on chromosome 8 at locations 22027873-22045326. The hairless gene, which similarly aids in histone demethylation, is a member of the JmjC domain superfamily. With 1189 residues in the hairless protein, the domain sequence spans positions 946 to 1157 and is 212 amino acids long. JmjC domains have been identified in over 100 bacterial and eukaryotic sequences due to significant sequence similarity. Among them the human hairless gene, which is mutated in alopecia universalis sufferers. We have attempted to use the bioinformatics method to homology model the JmjC domain in the hairless protein. The tools and programmes used in this work are NCBI-BLASTP, EBIClustalW, SMART, 3D-PSSM, DeepView/Switzerland-PDB Viewer, PyMOL, and WhatCheck. The structure of the JmjC domain is predicted using the template crystal structure of the probable antibiotic biosynthesis protein from Thermus thermophilus HB8. The minimised energy value of the modelled domain structure was -3394.570 KJ/mol. The WHAT IF-Proteins Model Check tool was used to verify the simulated domain structure.
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